I have tried the colony cracking method to screen for postive clones. The pYES260 plasmid size is 5933bp.I digested it with BamHI (1 site) and filled-in to create blunt end plasmid. My PCR insert is about 2kB. After electroporation and plating, I extracted the plasmid using colony cracking. However I am not sure which band represented my plasmid.
The first lane on the upper left is the pYES plasmid while other lane are my clones.
Is what I assumed correct?
- The pink line is the band for genomic DNA
- The blue line is where two bands were observed would be the RNA (Is the faint band across the lane is RNA?)
- The purple line is where the proteins were.
- The yellow line is the PLASMID.
Can anyone have a look at the gel picture attached?