Shift in the position of band onto the western blot
Posted 16 October 2012 - 10:13 PM
Posted 16 October 2012 - 11:33 PM
Denaturing gel or native?
Posted 17 October 2012 - 01:29 AM
Posted 17 October 2012 - 12:39 PM
Are you sure it isn't a cross-reacting band from the antibody (which is a very common phenomenon with antibodies, no matter how they are purified)?
Can you see the un-modified form in the same lane? Do you have a way of reliably making the higher band appear (and what is it)?