cell_bee, on 14 September 2012 - 12:33 PM, said:
I am a very new person to cell culture and would need some help with the calculations...
1. I am testing few inhibitors on mouse cellline. I have a chemical compound whose MW is 243. I need to dissolve it in DMSO and get the concentration to 10mM. Then I need to dilute it to 200uM and serial dilute this to 20uM-10um-5um-2.5um and so on...
How do I do this and when serial diluting it, do I have to use the growth media or do I use DMSO? I do not want the concentration of DMSO to go more than 1%. Please help
2. I have BMP4 whose concentration is 10ug/ml. I need 10ul of this, whose concentration is 250pM. How do I calculate?
what you know: MW= 243
what you want: 10mM , meaning 10 moles/m³ or 10^-2 moles/liter
so how to solve it?
243 grams/mole , you need 10 moles/m³ or 10^-2 moles/liter.
so if you want to make 1 liter, you need to take 10^-2 moles and you know its 243 grams/mole, so you should be able to figure out how many grams you need to take...
Are you sure you need such small amounts?
And no: you only dissolve it a bit of DMSO and add this to the growth medium. So if you make (for example) 1liter, you make 0,99 liter (for example) of growth medium with 0,01liter DMSO (not sure how well it will dissolve.. this depends .. so you need to try this yourself).
Same goes for the second queston; write down what you have and what you want... see how far you can come.
Edited by pito, 15 September 2012 - 07:34 AM.
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.