deletion of large fregments
Posted 16 October 2012 - 12:55 AM
I am using the quick-change method for site directed mutagenesis. so far, i was able to create single-base or codon change with no problem. now i need to delete a whole section of 20AA (60BP)
could this be preformed using the regular procedure? do i need special consideration when designing the primers?
Posted 16 October 2012 - 03:03 PM
Posted 19 October 2012 - 07:37 PM