I have a probe sonicator and am currently going up to 20 min of 2 second pulses in ice water to keep the temperature down to try this out....but does anyone have any prior experience or protocol specific to breaking proteins up? Are there other, better, ways to mechanically break a protein apart?
0
sonication to break apart a protein- is this feasible?
Started by Turing, Oct 15 2012 05:02 PM
sonication break protein
2 replies to this topic
#2
mdfenko
-
- Active Members
-
- 2,240 posts
an elder
77
Excellent
Excellent
Posted 19 October 2012 - 09:54 AM
are you talking about breaking an aggregate or are you talking about breaking the peptide chain?
sonication can disperse an aggregate. it's difficult to mechanically break peptide bonds.
sonication can disperse an aggregate. it's difficult to mechanically break peptide bonds.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
Turing
-
- Members
-
- 2 posts
member
0
Neutral
Neutral
Posted 19 October 2012 - 12:12 PM
I actually am trying to break open a compound composed of many subunits.....so I'm guessing it is something inbetween breaking aggregates and peptide bonds.
Also tagged with one or more of these keywords: sonication, break protein
 |
Protocols and Techniques Forums →
ChIP and Next Generation Sequencing →
Sonication Help!Started by Guest_j47027_* , 19 Apr 2013  Sonication, RNaseA, Proteinase K and 1 more...
|
|
|
|
Protocols and Techniques Forums →
ChIP and Next Generation Sequencing →
power and fragment length: how correlated are they?Started by Guest_SStamis_* , 31 Aug 2012 ChIP, DNA fragment, sonication and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Immunology →
ELISA and Immunoassay →
Can I test protein denaturation after sonicationStarted by Guest_venoest_* , 31 Aug 2012 sonication and 3 more...
|
|
|
||
Protocols and Techniques Forums →
ChIP and Next Generation Sequencing →
|
|
|
||
Protocols and Techniques Forums →
General Lab Techniques →
|
|
|
