2 replies to this topic

[ACN]

I need to order secondary antibodies!

So I’m working with Sendai virus, and I want to study the proteins produced during the early stages of an infection. I will be running a Western blot. My Primary antibody is (Rabbit) targeted against Sendai virus in general. I would like to know what would be the best Secondary antibody to purchase ?
I know there was pervious success with a: Goat anti-Rabbit IgG conjugated to HRP

A number of companies sell Goat anti-Rabbit IgG conj to HRP,  but, the companies also offer a wide range of target specificity (i.e. IgG (gamma) Fc, H+L, and so on…). Which should I use? or even better if someone could point me to a book or a link to website that explains what to use and when,, that would be great!

[casandra]

This: http://www.piercenet...e-second-Ab.pdf will give you a good overview.  We usually get our labelled secondaries from Jackson Immunoresearch...they are reliable and the prices are quite reasonable.

[ACN]

casandra, on 15 October 2012 - 01:07 PM, said:

This: http://www.piercenet...e-second-Ab.pdf will give you a good overview.  We usually get our labelled secondaries from Jackson Immunoresearch...they are reliable and the prices are quite reasonable.

Thanks, that link didn't seem to work for me; but, I'm finding some really useful information here: http://www.pierce-an...ary-antibodies/