Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Nocodazole-induced apoptosis

  • Please log in to reply
1 reply to this topic

#1 julnobody



  • Active Members
  • Pip
  • 18 posts

Posted 15 October 2012 - 05:54 AM


I have a death assay to perform quite quickly (7AAD) and I was planning to use Nocodazole (that i have in large quantity) as positive control over 24hrs. (on U87 glioma cells)

Is it possible and what concentration should I use ?

Thank you

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,116 posts

Posted 15 October 2012 - 11:30 AM

Here's what my go-to drug list has to say:

Has specific antimicrotubular activity for mammalian cells in culture. Promotes
microtubule depolymerization. Nanomolar concentrations alter microtubule dy-
namics and interfere with fibroblast locomotion without affecting polymer levels.
Arrests cells in mitosis.
Soluble in: DMSO
Stock concentration: 10 to 30 mM (store at room temperature)
Working concentration: 50 nM (low concentrations) to 30 µM (for effective mi-
crotubule depolymerization)
Duration of incubation: For rapid depolymerization of microtubules, preincubate
cells on ice with nocodazole for 15 min; use up to 24 hr.

Note: the actual concentration you should use will depend on the cell line and should be titrated before use.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.