Hi,
I have a death assay to perform quite quickly (7AAD) and I was planning to use Nocodazole (that i have in large quantity) as positive control over 24hrs. (on U87 glioma cells)
Is it possible and what concentration should I use ?
Thank you
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bob1
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Thelymitra pulchella
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Posted 15 October 2012 - 11:30 AM
Here's what my go-to drug list has to say:
Note: the actual concentration you should use will depend on the cell line and should be titrated before use.
Quote
Nocodazole
Has specific antimicrotubular activity for mammalian cells in culture. Promotes
microtubule depolymerization. Nanomolar concentrations alter microtubule dy-
namics and interfere with fibroblast locomotion without affecting polymer levels.
Arrests cells in mitosis.
Soluble in: DMSO
Stock concentration: 10 to 30 mM (store at room temperature)
Working concentration: 50 nM (low concentrations) to 30 µM (for effective mi-
crotubule depolymerization)
Duration of incubation: For rapid depolymerization of microtubules, preincubate
cells on ice with nocodazole for 15 min; use up to 24 hr.
Has specific antimicrotubular activity for mammalian cells in culture. Promotes
microtubule depolymerization. Nanomolar concentrations alter microtubule dy-
namics and interfere with fibroblast locomotion without affecting polymer levels.
Arrests cells in mitosis.
Soluble in: DMSO
Stock concentration: 10 to 30 mM (store at room temperature)
Working concentration: 50 nM (low concentrations) to 30 µM (for effective mi-
crotubule depolymerization)
Duration of incubation: For rapid depolymerization of microtubules, preincubate
cells on ice with nocodazole for 15 min; use up to 24 hr.
Note: the actual concentration you should use will depend on the cell line and should be titrated before use.
