I want to label my protein (platelet integrin 2b3a) with an amine reactive dye. The protein comes in Tris buffer (pH 7.4) which I need to replace with 0.1M sodium bicarbonate buffer at pH8.3. To do this I was going to dialyse the protein against the new buffer. Will the protein be ok for 24 hours or more in a pH8.3 buffer with 0.1 sodium bicarbonate?
Edited by Wyanstown, 15 October 2012 - 04:38 AM.