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Linearising plasmid before transfection?


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2 replies to this topic

#1 science noob

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Excellent

Posted 14 October 2012 - 10:22 PM

Is there a need to linearise a circular plasmid DNA before transfecting it into a mammalian cell with Lipofectamine or electroporation? Or the circular form will integrate into the host cell automatically?

#2 Curtis

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Excellent

Posted 14 October 2012 - 11:34 PM

http://www.protocol-...posts/6156.html

http://www.protocol-.../6793more1.html

http://www.protocol-...posts/5619.html

http://ko.cwru.edu/p...avesNotes3.html

#3 cellthetruth

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Neutral

Posted 15 October 2012 - 11:22 PM

There is no need to linearise. But if you do, the advantage is that you don't disrupt your gene, promoter and resistance.




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