2 replies to this topic

[science noob]

Is there a need to linearise a circular plasmid DNA before transfecting it into a mammalian cell with Lipofectamine or electroporation? Or the circular form will integrate into the host cell automatically?

[Curtis]

http://www.protocol-...posts/6156.html

http://www.protocol-.../6793more1.html

http://www.protocol-...posts/5619.html

http://ko.cwru.edu/p...avesNotes3.html

[cellthetruth]

There is no need to linearise. But if you do, the advantage is that you don't disrupt your gene, promoter and resistance.