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Linearising plasmid before transfection?
2 replies to this topic
Posted 14 October 2012 - 10:22 PM
Is there a need to linearise a circular plasmid DNA before transfecting it into a mammalian cell with Lipofectamine or electroporation? Or the circular form will integrate into the host cell automatically?
Posted 15 October 2012 - 11:22 PM
There is no need to linearise. But if you do, the advantage is that you don't disrupt your gene, promoter and resistance.