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U937 and differentation to macrophages


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#1 MariaDomenica

MariaDomenica

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Posted 12 October 2012 - 12:36 AM

Hi!
I´m starting to work with this cell and so to induce them to macrophages...my question is really sample:
You differentiate them in a flask or direct to the well plate?
What is for you the best desity of the cell to use?
I used 5X10^5 cells/ml with different concentration of PMA...5nM;10nM;15nM;20nM...but at 25 and 20nM they died,,,,




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