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ura3 mutant yeast strain grows on SD-uracil-

ura3 SD -uracil yEplac195

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#1 Yunus Ensari

Yunus Ensari


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Posted 11 October 2012 - 04:29 AM


ı have a yeast strain (W303-1A) which is ura3 mutant. thus ıt does not grow on SD-uracil medium.

two days ago first I inoculated to YPAD medium and incubated at 28 degree overnight. after that ı used this culture for inoculation to SD - uracil medium. after overnight incubation the yeast growth.

what is the problem. Do you have any idea about this case.


#2 phage434



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Posted 11 October 2012 - 04:49 AM

Three possibilites:
1) Your medium is not really ura-
2) Your yeast is not ura-
2a) A rare but possible mutation restored ura+ to your strain.

I'd vote for a contaminant ura+ strain, but check the medium with a verified ura- strain.

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