Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

ura3 mutant yeast strain grows on SD-uracil-

ura3 SD -uracil yEplac195

  • Please log in to reply
1 reply to this topic

#1 Yunus Ensari

Yunus Ensari

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 11 October 2012 - 04:29 AM

hello,

ı have a yeast strain (W303-1A) which is ura3 mutant. thus ıt does not grow on SD-uracil medium.

two days ago first I inoculated to YPAD medium and incubated at 28 degree overnight. after that ı used this culture for inoculation to SD - uracil medium. after overnight incubation the yeast growth.

what is the problem. Do you have any idea about this case.

thanks.

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,475 posts
251
Excellent

Posted 11 October 2012 - 04:49 AM

Three possibilites:
1) Your medium is not really ura-
2) Your yeast is not ura-
2a) A rare but possible mutation restored ura+ to your strain.

I'd vote for a contaminant ura+ strain, but check the medium with a verified ura- strain.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.