Amplicons detected for negative control sites (ChiP-qPCR)
Posted 10 October 2012 - 09:58 AM
1) what are the normal Ct values one would expect to see with negative site primers? I was expecting around 30-35 (or higher) since in theory they should not be amplifying anything?
2) what is the typical/acceptable fold change that would work well for the hi-seq?
Appreciate any help given. Thanks!
Posted 27 November 2014 - 10:29 PM
I think as long as you have more then 5 fold difference between positive and negative regions, it should be ok