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We have been growing primary human sinonasal epithelial cells in trans-wells at p2, until confluence. They are grown to confluence in BEGM (Lonza), and switched to a 50:50 mixture of BEGM and DMEM with an addition of Retinol placed in the basolateral side of the trans-well. Theoretically, these cultures should be able to last for about 6 months, but we have experienced considerable leaching about a month and a half into the ALI. We tried making a new batch of Retinol, but are still experiencing leaching. Has anyone else struggled with this, and if so what did you do about it?
Thank you!
Edited by wendy245, 10 October 2012 - 09:00 AM.
