16 replies to this topic

[Mad researcher]

Do you guys suggest preparing a master mix without the TAQ and storing it at -20 for further use?

[phage434]

If you store in Tris or (worse) water, you are at the mercy of accidental DNAse contamination.  This is why TE is so much better.  And the idea that 1 mM EDTA compromises an enzyme reaction when the amount added as primers is typically 5% of the final solution is quite absurd.