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Primers Freeze-Thaw

primers

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16 replies to this topic

#16 Mad researcher

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Posted 12 October 2012 - 07:19 AM

Do you guys suggest preparing a master mix without the TAQ and storing it at -20 for further use?
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Mad Researcher

#17 phage434

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Posted 12 October 2012 - 01:19 PM

If you store in Tris or (worse) water, you are at the mercy of accidental DNAse contamination. This is why TE is so much better. And the idea that 1 mM EDTA compromises an enzyme reaction when the amount added as primers is typically 5% of the final solution is quite absurd.





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