I'm not sure how and how long it is possible to store a PVDF membane after protein transfer but before addition of the primary antibody.
The problem is that I've ordered a new antibody and delivery will take some days. So is it possible to do the blot/transfer in advance, store the membrane and then add the primary antibody few days later? If yes, what are the best storage conditions? I've read to keep a membrane in blocking solution at 4 °C but I also found the information that extensive blocking is not recommended...
Does anyone have some tips?
Edited by 2xzwei, 08 October 2012 - 01:15 AM.