SODIUM BORATE BUFFER
20 g NaOH
pH to 8.0 with approx 120 g H3BO3 (boric acid powder)
bring to 1 L with dH2O
NOTE: This stock will precipitate over time, requiring heat to re-dissolve crystallized salts before dilution.
1x Working Buffer:
20 ml of 50x stock
980 ml dH2O
NOTE: The pH of the working solution will be higher than that of the 50x stock
NOTE: Do not re-use 1x solution for more than a day or it will go fuzzy. Really.
Translated to concentrations:
50x Stock: 500 mM NaOH, pHed to 8.0 w/ H3BO3
1x Working buffer: 10 mM NaOH, pHed to 8.5 w/ H3BO3
Just removed your duplicated post bits. Bob