Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

SB buffer recipe.

Sodium borate buffer SB buffer

  • Please log in to reply
5 replies to this topic

#1 Mad researcher

Mad researcher

    Researcher in making

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 105 posts
7
Neutral

Posted 07 October 2012 - 10:20 AM

Is this recipe for SB buffer correct? i found it online. If not, can you give me the correct recipe please?


SODIUM BORATE BUFFER



50x Stock:

20 g NaOH
pH to 8.0 with approx 120 g H3BO3 (boric acid powder)
bring to 1 L with dH2O

NOTE: This stock will precipitate over time, requiring heat to re-dissolve crystallized salts before dilution.


1x Working Buffer:

20 ml of 50x stock
980 ml dH2O

NOTE: The pH of the working solution will be higher than that of the 50x stock
NOTE: Do not re-use 1x solution for more than a day or it will go fuzzy. Really.


Translated to concentrations:

50x Stock: 500 mM NaOH, pHed to 8.0 w/ H3BO3
1x Working buffer: 10 mM NaOH, pHed to 8.5 w/ H3BO3

Just removed your duplicated post bits. Bob
Cheers,

Mad Researcher

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,713 posts
398
Excellent

Posted 07 October 2012 - 12:25 PM

Those look correct to me.

I re-use SB several times, sometimes even over a month or so I will use the same buffer. find it slows down after about 5x reuse.

#3 Mad researcher

Mad researcher

    Researcher in making

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 105 posts
7
Neutral

Posted 08 October 2012 - 04:04 AM

I like the SB buffer and i have replaced my TBE buffer with SB buffer. It was quite fast :-)
Cheers,

Mad Researcher

#4 Mad researcher

Mad researcher

    Researcher in making

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 105 posts
7
Neutral

Posted 12 October 2012 - 06:59 AM

What voltage do you prefer while using SB buffer and for how much time?
Cheers,

Mad Researcher

#5 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,713 posts
398
Excellent

Posted 12 October 2012 - 07:28 PM

Depends on the size of the product I am looking for. Typical PCR of 500 ish bp, and I would go 300 V for 6 -10 min.

#6 Mad researcher

Mad researcher

    Researcher in making

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 105 posts
7
Neutral

Posted 12 October 2012 - 11:48 PM

I have 300 bp products and i run it at 150 V for 40 mts.
I am planning to increase the voltage to 250 V and reduce the time to 20 mts.
Cheers,

Mad Researcher




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.