dilution of cell suspension
Posted 07 October 2012 - 05:52 AM
I want to seed a 96 well plate from a T25 flask having a volume of 4mL. Each well should have 10,000 cells in 100uL. I trypsinised the cells and added 1mL of medium to get a cell suspension. Its concentration is 1e6 cells/mL. So I took the entire 1mL of cell suspension and diluted it with 9mL of medium. From this I added 100uL into each well so that it contains 10,000 cells in 100 uL. Is this correct?
Posted 07 October 2012 - 12:29 PM
Posted 09 October 2012 - 08:34 AM
Posted 09 October 2012 - 12:22 PM