Im doing DNA extraction for MCF-7 and HeLa cells. They have undergo drug treatment and I just want to look at their DNA fragmentation (if any). The problem is, when I wash them with EtOH, centrifuge, dry the EtOH, i suppose to have a pellet or perhaps some debris on my tube. but instead of that, i have a bulk of gooey sticky stuff.. i discover that the gooey sticky stuff is the DNA because when i run gel, they all have bands. but unfortunately, with some RNA below.
i hope to know is there any possibility to reduce the viscosity of that gooey stuff and how can i eliminate the protein content in that gooey stuff? i have read alot of discussion here. but most of them is about how to eliminate the DNA and getting the protein.
Edited by JustCurious, 06 October 2012 - 07:34 PM.