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Compound to Differentiate Monocytes

Monocyte Macrophage phagocyte immunology tissue culture

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#1 MycoZ

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Posted 04 October 2012 - 12:38 PM

Hi.

At the risk of being redundant from my previous post....

Does anyone work with Mono-Mac-1 cells? If not, how about the sister cell line Mono-Mac 6? I need to differentiate the cells into MDMs. What should I use to do that? I see in the literature people either use LPS or PMA, although not that many people seem to work with this. What about IFN-gamma? I know that's used for other cell lines, like U937.

Thanks!

#2 CMIRC

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Posted 25 October 2012 - 05:20 AM

Hello

I have not worked with the cell line you are working with, but with THP1 monocytes I have previously got differentiation to macrophages within 3 days of incubation with 1uM PMA. Hope this is helpfull. Sorry if it does not answer your question.





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