I'm using northern blot for my research & I'd like to try probing with my target probe as well as a probe for a housekeeping gene (actin). Should this be done separately or can it be done at the same time (ie. save time & materials)?
northern blot with two probes
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Posted 17 November 2003 - 11:27 AM
You can certainly probe a Northern blot with two -or more - probes at once - provided that they will show up at different enough molecular weights. You may want to consider running a somewhat longer gel than usual to improve seperation.