Question about fixation and washfixation and wash
Posted 02 October 2012 - 10:18 PM
Posted 03 October 2012 - 01:23 PM
Of note - if you are talking about centrifuges, rpm is not helpful as the force applied by the centrifuge is dependent on the radius of the rotor - you need to refer to RCF or gravity ("g") forces
Posted 18 October 2012 - 06:51 AM
In case you are fixing the cells and have to really do all the spins, try going for 300G for 10 minutes. Once fixed, it is easy to lose cells and so a longer spin time might get them pelleted. I never go lower than 1500 rpm and 6-7 minutes even when I am working with cells that are not fixed.