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Renilla:Firefly:miRNA ratios


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#1 Rawraw

Rawraw

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Posted 02 October 2012 - 06:52 AM

Hello,

I am trying to titrate the ratio of luciferase vectors(Renilla:firefly) to miRNA. I know I need to find the ratio that will knockdown signal as much as possible. However, how do I assess what that low signal should be? Is there some sort of baseline to which I should compare?

Thank you,

Rawraw

Edited by Rawraw, 02 October 2012 - 10:08 AM.





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