I am trying to titrate the ratio of luciferase vectors(Renilla:firefly) to miRNA. I know I need to find the ratio that will knockdown signal as much as possible. However, how do I assess what that low signal should be? Is there some sort of baseline to which I should compare?
Thank you,
Rawraw
Edited by Rawraw, 02 October 2012 - 10:08 AM.













