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SF9 transfection with siRNA - what transfection reagent has worked for you?

cell culture

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3 replies to this topic

#1 Mai Doan

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Posted 02 October 2012 - 06:44 AM

I am working on transfecting a few different siRNA into sf9 cells. I am wondering if anyone else has ever done this and what transfection reagent works best for you? I am going to be using TransMessenger Transfection Reagent from Qiagen, but would love to get input on other products that might be better than the one I am using.

Thank you,

Mai

#2 bob1

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Posted 02 October 2012 - 12:42 PM

I've had good success with RNAiMax from Life Technologies/Invitrogen.

#3 Mai Doan

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Posted 03 October 2012 - 12:54 PM

Thank you bob1 for the tip. You used RNAiMax for sf9 cells right? What were your transfection efficiency? I will look into it and try.

#4 bob1

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Posted 03 October 2012 - 01:04 PM

No, I didn't use it for SF9 cells, but it shouldn't make too much difference. I have used it in about 10 different immortal cell lines though. I didn't make any direct measurement of the transfection efficiency but was able to see 90+% KD of the RNA and a similar level of the protein.





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