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Brief vortexing for reconstitution of recombinant proteins?

dissolving proteins

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#1 gunes

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Posted 27 September 2012 - 12:22 AM

Hello,

I have a very simple question.

Generally, vortexing is not recommended while reconsituting lyophilized recombinant proteins.

Can a brief vortexing (e.g. 10 sec. in 1xPBS supplemented with 0.1% BSA) result in a significant loss of the proteins' bioactivity? Did anyone experience problems because of vortexing?

Gunes.


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#2 mdfenko

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Posted 27 September 2012 - 11:43 AM

vortexing can cause denaturation of the protein due to physical stress (and also, possibly, oxidation).

it can be performed if you can cause a swirling effect rather than bumping.
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