Hello,
I have a very simple question.
Generally, vortexing is not recommended while reconsituting lyophilized recombinant proteins.
Can a brief vortexing (e.g. 10 sec. in 1xPBS supplemented with 0.1% BSA) result in a significant loss of the proteins' bioactivity? Did anyone experience problems because of vortexing?
Gunes.
- goals go global -
0
Brief vortexing for reconstitution of recombinant proteins?
Started by gunes, Sep 27 2012 12:22 AM
dissolving proteins
1 reply to this topic
#2
mdfenko
-
- Active Members
-
- 2,240 posts
an elder
77
Excellent
Excellent
Posted 27 September 2012 - 11:43 AM
vortexing can cause denaturation of the protein due to physical stress (and also, possibly, oxidation).
it can be performed if you can cause a swirling effect rather than bumping.
it can be performed if you can cause a swirling effect rather than bumping.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
