Hi all,
Just wanted to know that if I plan to use cDNA sequencing to determine the sequence of a particular gene, how can we determine the sequence of the complete length of the cDNA. Doesn't common sequencing have limits of 600-800 bp? So, if my cDNA is say 1600 bp long, how can I determine the sequence of the other 800 bp? Or is it that other methods of sequencing are employed in such cases.
Thanks for your help.
Ameya
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2 replies to this topic
#1
Ameya P
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Rervm Cognoscere Cavsas
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Posted 26 September 2012 - 10:43 PM
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Replication of this art is strictly prohibited without express permission of the artist
#2
Trof
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Brain on a stick
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Posted 27 September 2012 - 01:45 PM
The method is called "design additional sequencing primer in the middle of your sequence" 
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
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#3
bob1
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Thelymitra pulchella
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Posted 27 September 2012 - 02:07 PM
You can also try 5' and 3' RACE.
