Hi all, I am trying to clone an already made construct (E3519 plasmid + EGFP) onto GV3101 agrobacterium. After the transformation, I try to extract plasmids using Qiagen kit but I don't get any plasmid or I get very low conc and when I run on gel I don't see any band. Please can someone help me identify what the problem is? Also, can anyone give me a standard protocol for agrobacterium transformation? Thanks.
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