Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Agrobacterium transformation


  • Please log in to reply
No replies to this topic

#1 och

och

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 26 September 2012 - 07:08 PM

Hi all, I am trying to clone an already made construct (E3519 plasmid + EGFP) onto GV3101 agrobacterium. After the transformation, I try to extract plasmids using Qiagen kit but I don't get any plasmid or I get very low conc and when I run on gel I don't see any band. Please can someone help me identify what the problem is? Also, can anyone give me a standard protocol for agrobacterium transformation? Thanks.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.