Hello Everyone,
I am trying to clone a 3.6 KB gene from the cDNA using a GSP. I am using the Superscript III enzyme from invitrogen and they specify using 2 pmol of GSP. So I made a 0.1 micromolar stock and used 1 microlitre for priming the cdna synthesis rxn. However the gene did not amplify. (I am going to use internal primers to check if atleast 1-2 kb of the gene amplified!) Does anyone have any experience or recommendations for using a particular primer concentration which works?
Thanks in Advance,
0
Primer concentration for cDNA synthesis using GSP
Started by PlantS_Student, Sep 26 2012 09:59 AM
cdna cloning GSP primer
1 reply to this topic
Also tagged with one or more of these keywords: cdna, cloning, GSP, primer
 |
Protocols and Techniques Forums →
Molecular Cloning →
Too many negative clones using Directional TOPO CloningStarted by Guest_zbiology_* , Yesterday, 07:02 PM  Ligation, cloning, Invitrogen
|
|
|
|
|
|
Protocols and Techniques Forums →
Genetics and Genomics →
Design construct for promoter activity assayStarted by Guest_DrLeo_* , Yesterday, 05:34 AM promoter activity, Cloning
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
"Easiest" sticky end combinationStarted by Guest_doxorubicin_* , 21 May 2013 ligation, cloning and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Reproducible Non-Specific PCR ProductStarted by Guest_Epigeneticist_* , 20 May 2013 PCR, Sequencing, Cloning
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
classic cloning with restriction enzymes into empty pcDNA3.1DStarted by Guest_Euterpa12_* , 17 May 2013 cloning, pcDNA3.1
|
|
|
