Hello Everyone,
I am trying to clone a 3.6 KB gene from the cDNA using a GSP. I am using the Superscript III enzyme from invitrogen and they specify using 2 pmol of GSP. So I made a 0.1 micromolar stock and used 1 microlitre for priming the cdna synthesis rxn. However the gene did not amplify. (I am going to use internal primers to check if atleast 1-2 kb of the gene amplified!) Does anyone have any experience or recommendations for using a particular primer concentration which works?
Thanks in Advance,
0
Primer concentration for cDNA synthesis using GSP
Started by PlantS_Student, Sep 26 2012 09:59 AM
cdna cloning GSP primer
1 reply to this topic
Also tagged with one or more of these keywords: cdna, cloning, GSP, primer
 |
Protocols and Techniques Forums →
Molecular Cloning →
classic cloning with restriction enzymes into empty pcDNA3.1DStarted by Guest_Euterpa12_* , 17 May 2013  cloning, pcDNA3.1
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Cloning large fragmentsStarted by Guest_bknm_* , 15 May 2013 Cloning, PCR, large inserts
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Transformation colonies does not contain insertStarted by Guest_Sandy Fowler_* , 08 May 2013 transformation, Ligation, Cloning
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Primer Efficiency across runsStarted by Guest_Joe101_* , 08 May 2013 PCR, Primer, RT-PCR, Efficiency and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Need urgent advise..Started by Guest_M.T2013_* , 29 Apr 2013 cloning
|
|
|
