Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

protocol for Measuring Gluconeogenesis using deuterium water

gluconeogenesis deuterium water deuterium oxide

  • Please log in to reply
No replies to this topic

#1 JamieSmith88



  • Members
  • Pip
  • 1 posts

Posted 25 September 2012 - 01:09 AM


I am looking for detailed protocols to measure gluconeogenesis using deterium water and totoal hepatic glucose production using [2H 2]- glucose in humans. The papers that I have read do not give enough information in order to actually do the experiments.

briefly, the method that i want to use is for volunteers to ingest deuterium water and infuse [2H 2]- glucose and do penta acetate derivatives of the plasma glucose samples. These will then be measures by GC mass spec to determine deuterium enrichment at specific carbons eg C5.

Please let me know if you have any idea where I can get hold of such protocols.


Also tagged with one or more of these keywords: gluconeogenesis, deuterium water, deuterium oxide

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.