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culture media for pancreatic cancer stem cells


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#1 mihoz

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Posted 24 September 2012 - 10:59 PM

I am planning to isolate pancreatic cancer stem cells and test on them a couple of drugs. However, i am not so sure of the exact media that I'd use to propagate the cells without differentiation. (i'll be testing the drugs in sphere formation assays & migration assays).

I know the media has to be serum-free, have growth factors: EGF & FGF-2.

Does anyone have a recipe?
thanks a lot

#2 mihoz

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Posted 24 September 2012 - 11:03 PM

I am planning to isolate pancreatic cancer stem cells and test on them a couple of drugs. However, i am not so sure of the exact media that I'd use to propagate the cells without differentiation. (i'll be testing the drugs in sphere formation assays & migration assays).

I know the media has to be serum-free, have growth factors: EGF & FGF-2.

Does anyone have a recipe?
thanks a lot

#3 mihoz

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Posted 24 September 2012 - 11:06 PM

I am planning to isolate pancreatic cancer stem cells and test on them a couple of drugs. However, i am not so sure of the exact media that I'd use to propagate the cells without differentiation. (i'll be testing the drugs in sphere formation assays & migration assays).

I know the media has to be serum-free, have growth factors: EGF & FGF-2.

Does anyone have a recipe?
thanks a lot

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