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PCR & Southern blotting

southern hybridization PCR

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#1 uday

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Posted 24 September 2012 - 05:43 PM

How to justify the validation with southern hybridization over PCR amplification for a specific gene detection ? I know this is a conventional question but still need to look for a better clarification.

#2 bob1

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Posted 25 September 2012 - 12:17 AM

Typically Southern blots are more specific as you use a long probe to detect the gene, however, the technique is relatively low sensitivity. PCR has lower specificity but much higher sensitivity.





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