Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Cleaning/eliminating E.coli adsorbed onto substrates


  • Please log in to reply
1 reply to this topic

#1 newbbio

newbbio

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 24 September 2012 - 06:21 AM

What are methods of completely cleaning and eliminating all E.coli cells that are adsorbed onto various substrates, such as glass or other materials? Ideally there should be zero residue.

Would a long duration protease wash (such as trypsin) be good enough?

What about ultra high temperature heating for substrates that can sustain the temperature? E.g. would heating e.coli to 600C completely thermally decompose and vaporize the contents of E.coli? Would it leave any residue (such as pyrolytic compounds or salts)?

Thank you/

#2 ascacioc

ascacioc

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 271 posts
44
Excellent

Posted 24 September 2012 - 12:25 PM

I would autoclave + piranha solution (the H2SO4 + hydrogen peroxide solution)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.