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In vitro transcription/translated protein to cells!!!

In vitro transcription In vitro translation

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#1 dvpr2012

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Posted 23 September 2012 - 02:24 PM

Hello,

I am trying to do In vitro transcription/translation using reticulocyte lysate. Do you know if the protein produced using this method can be used for treating/culture with cells. I want the protein to enter the cells. Is it possible or has anyone around tried it??

#2 ascacioc

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Posted 24 September 2012 - 12:45 PM

If you have a tag, like His-tag on the translated protein, I would do a quick purification using a bit of Ni-NTA beads (or whatever appropriate according to tag). Followed by a desalting on a concentrator column to get rid of the imidazole. And then filter sterilize the outcome to be sure you are not contaminating your cells.





Also tagged with one or more of these keywords: In vitro transcription, In vitro translation

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