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Primer Design HELP!

Started by Eriel Lualhati, Sep 23 2012 09:30 AM

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3 replies to this topic

#1 Eriel Lualhati

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Posted 23 September 2012 - 09:30 AM

Hi guys,
Is there a posibility of making primers that are designed for amplification of Beta Actin Gene in both Cauliflower and Chicken liver DNA?

Please read the attachment >.<

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#2 pcrman

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Posted 23 September 2012 - 10:22 AM

It is possible if the two species happen to share a common sequence for beta actin gene.

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#3 Trof

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Posted 23 September 2012 - 11:56 AM

AFAIK there is no known sequence for beta actin gene of Brassica oleracea.
Don't see any attachement.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

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#4 ascacioc

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Posted 24 September 2012 - 11:39 AM

align the two sequences and hope that both the 5' and the 3' are conserved for the first 15-20 bp and last 15-20 bp; this if you want the full sequence start to end