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Primer Design HELP!


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Poll: Primer Design HELP! (0 member(s) have cast votes)

Is it possible?

  1. Yes (0 votes [0.00%])

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  2. No? (0 votes [0.00%])

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#1 Eriel Lualhati

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Posted 23 September 2012 - 09:30 AM

Hi guys,
Is there a posibility of making primers that are designed for amplification of Beta Actin Gene in both Cauliflower and Chicken liver DNA?

Please read the attachment >.<

#2 pcrman

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Excellent

Posted 23 September 2012 - 10:22 AM

It is possible if the two species happen to share a common sequence for beta actin gene.

#3 Trof

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Excellent

Posted 23 September 2012 - 11:56 AM

AFAIK there is no known sequence for beta actin gene of Brassica oleracea.
Don't see any attachement.

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#4 ascacioc

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Excellent

Posted 24 September 2012 - 11:39 AM

align the two sequences and hope that both the 5' and the 3' are conserved for the first 15-20 bp and last 15-20 bp; this if you want the full sequence start to end




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