Problems with having to run the gels for so long
Posted 23 September 2012 - 09:06 AM
Posted 23 September 2012 - 12:07 PM
Your power source can be bad, your electrodes/tank can be bad. Try to change both. Does your gel heat during run even on low voltage? That could mean increased resistance through bad wiring/electrodes, gel or buffer. If the gel is running slow and is cool, and also bands are diffused, you may just not get enough current because of malfunctioning power source.
If you change both tank and power source and it's still slow, try to find problems in buffer and gel composition.
No more ideas for now.
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Posted 23 September 2012 - 12:21 PM
Posted 23 September 2012 - 12:52 PM
Posted 23 September 2012 - 12:53 PM
Posted 24 September 2012 - 12:32 PM
Posted 24 September 2012 - 12:51 PM
Posted 24 September 2012 - 12:54 PM
mA should not be 1 mA. I am not familiar with your gel system to tell you exactly. But you shouldn't get too low current. Check and tell me how much you get. For your gel composition I would expect smth around 10 mA (but I might be wrong)
Posted 24 September 2012 - 12:55 PM
Find a multimeter or something that can measure resistance - touch the probes to each end of the electrodes and/or leads and read the reisitance - if it approaches 0, then they are fine.
how do I check the electrodes, and what should the mA be around?
Short circuiting will lead to very high current.
Posted 24 September 2012 - 12:59 PM
Your current should not be 1 mA and not too much. Now, it would have been nice for you to know what the current should be from a nice run. Next time when you have a nice run, check the current and note it down. It might be handy to know it for next time when you have troubles.
Posted 24 September 2012 - 01:08 PM
Posted 24 September 2012 - 01:14 PM
Do you have the same phenomenon in both gel aka not running? Then it is the power pack or smth else that is in common for both gels. Did you redo the buffer?
Posted 24 September 2012 - 01:20 PM
Posted 24 September 2012 - 01:26 PM
Posted 27 September 2012 - 02:26 PM