Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

MTT with and without serum

  • Please log in to reply
1 reply to this topic

#1 SF_HK



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 231 posts

Posted 23 September 2012 - 07:59 AM


Why is MTT performed on cells growing in serum free medoum (SFM) and in medium with serum. Why not just on medium with serum?

#2 ascacioc



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 271 posts

Posted 24 September 2012 - 11:36 AM

Depends on the cell line. In a lab I was doing MTT some years ago we were doing it always with serum, except one student that had to starve the cells without serum because her cells were hormone responsive and she wanted to see the effect with and without the hormones present. Serum starvation is a common way to remove the hormonal effect i.e. if you have thyroid cells or prostate cancer cells, you do not want to the state of the cells when the specific pathway is already activated by the hormone.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.