For expressing protein in non-E.coli cells which plasmid can be used? thanks.
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#1
prabhubct
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Posted 22 September 2012 - 02:09 AM
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#2
Trof
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Posted 22 September 2012 - 02:31 AM
Well you need to know first what cells you're going to use, then you can find suitable vector.
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#3
prabhubct
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Posted 22 September 2012 - 03:30 AM
Trof, on 22 September 2012 - 02:31 AM, said:
Well you need to know first what cells you're going to use, then you can find suitable vector.
Salmonella typhimurium. pcdna3.1 may be compatible but I need BamHI-SalI MCS containing vector. I do not need vector constitutive expression in a variety of mammalian cell lines. I'm looking for vector surviving in salmonella. like PET32 in E.coli.
Edited by prabhubct, 22 September 2012 - 03:45 AM.
“Those who mind don't matter, and those who matter don't mind.”
-- Bernard M. Baruch
-- Bernard M. Baruch
