Expressing protein in non-E.coli
Posted 22 September 2012 - 02:09 AM
Posted 22 September 2012 - 02:31 AM
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon
Posted 22 September 2012 - 03:30 AM
Well you need to know first what cells you're going to use, then you can find suitable vector.
Salmonella typhimurium. pcdna3.1 may be compatible but I need BamHI-SalI MCS containing vector. I do not need vector constitutive expression in a variety of mammalian cell lines. I'm looking for vector surviving in salmonella. like PET32 in E.coli.
Edited by prabhubct, 22 September 2012 - 03:45 AM.