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Expressing protein in non-E.coli


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#1 Inbox

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Posted 22 September 2012 - 02:09 AM

For expressing protein in non-E.coli cells which plasmid can be used? thanks.

#2 Trof

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Posted 22 September 2012 - 02:31 AM

Well you need to know first what cells you're going to use, then you can find suitable vector.

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Posted 22 September 2012 - 03:30 AM

Well you need to know first what cells you're going to use, then you can find suitable vector.


Salmonella typhimurium. pcdna3.1 may be compatible but I need BamHI-SalI MCS containing vector. I do not need vector constitutive expression in a variety of mammalian cell lines. I'm looking for vector surviving in salmonella. like PET32 in E.coli.

Edited by prabhubct, 22 September 2012 - 03:45 AM.





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