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Competitive vs. Sandwich ELISA


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#1 Ventilator

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Posted 21 September 2012 - 11:21 AM

Hi!

Due to which reason(s) is a competitive ELISA more sensitive than a sandwich ELISA?

Thank you!




(Source: e.g. http://www.ncbi.nlm....CEPT_AND_METHOD)

#2 bob1

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Posted 21 September 2012 - 11:20 PM

Hmmm... homework...

#3 Ventilator

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Posted 22 September 2012 - 03:50 AM

?

#4 bob1

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Posted 22 September 2012 - 03:25 PM

The general rule with homework is that you need to provide some sort of answer so that we can help you learn, rather than us just providing an answer.

#5 Ventilator

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Posted 23 September 2012 - 03:22 AM

This is not a "homework".

#6 bob1

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Posted 23 September 2012 - 12:28 PM

Still - it'll help you learn if you can think about the problem and tell us what you think, then we can provide expansion and/or corrections to your thoughts.

#7 Sotirios

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Posted 25 September 2012 - 12:36 AM

A competitive immunoassay is more sensitive to matrix issues. In contrast to sandich, in copetitive elisa the matrix effect contributes to the increasment of sensitivity during the competition step.
Athanasiou Sotirios




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