No replies to this topic

[clementine]

Hello,

I've been trying to sort a cell population (from an in vivo cell suspension) that expresses LacZ with a nuclear localization signal (nls) under the promoter of a marker gene.
Initially I tried FluoReporter kit (  FluoReporterKit.pdf   132.59K   85 downloads) from Invitrogen, but I suspect that the fluorogenic B-galactosidase substrate from the kit is not making it to the nuclei with the hypotonic shock. This protocol would not work in fixed cells since the substrate will diffuse without giving any signal (I tried that).

Would anybody have any suggestion (other kit, procedure)?

Thank you!