DNA lost dramatically during storage
Posted 20 September 2012 - 01:51 PM
I wonder what cause it drop so fast? May it be endonuclease contamination? If so, I think the cleaved DNA should still show absorb at 260nm.
May it because DNA attached to tubes? I use polypropylene tubes.
Please give me some suggestions. Thanks a lot!
Posted 20 September 2012 - 02:39 PM
Posted 20 September 2012 - 04:44 PM
Posted 21 September 2012 - 06:11 AM
try mixing or vortexing and read absorbance again. maybe it precipitated. what is your blank?
Thanks! I blanked with Tris, which I used to resolve DNA.
As my samples are genomic DNA, will it get sheared if I vortex it? I did mixed with finger flip though.
Posted 21 September 2012 - 06:12 AM
I'd strongly recommend storing your DNA in TE rather than in Tris buffer.
I read sometines EDTA interfere with PCR assay. I wonder if it is safe to use TE for qPCR? Thanks!
Posted 21 September 2012 - 07:47 AM