Guys,
has anyone worked with HEPG2 before for extraction of RNA
Does it needs B-ME addition to lysis buffer (contains alot of RNAse)
how ug of RNA can be obtained from 1X105
Does any one know???
Thanks guys in advance
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1 reply to this topic
#2
bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 20 September 2012 - 01:12 AM
If you are using trizol or phenol to extract, you shouldn't need any RNase inhibitors. However, havn't worked to HepG2 for RNA though.
Also tagged with one or more of these keywords: RNase, RNA ug, HepG2
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