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Extraction of RNA from HEPG2

RNase RNA ug HepG2

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#1 madelingirly

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Posted 19 September 2012 - 10:29 PM

Guys,
has anyone worked with HEPG2 before for extraction of RNA
Does it needs B-ME addition to lysis buffer (contains alot of RNAse)
how ug of RNA can be obtained from 1X105
Does any one know???
Thanks guys in advance

#2 bob1

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Posted 20 September 2012 - 01:12 AM

If you are using trizol or phenol to extract, you shouldn't need any RNase inhibitors. However, havn't worked to HepG2 for RNA though.





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