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PCR amplification of the terminal repeat


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#1 discovery

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Posted 28 February 2002 - 07:20 AM

one of my PCR primer is located in the terminal repeat region and I have trouble in the amplification.

Anyone that have good ideas?


#2 sean

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Posted 15 March 2002 - 04:25 PM

terminal repeat of what?

r u sayign u have repeats in ur constructs? or???

perhaps u can change the primers... that's one way.  if u must use the repeats, u will always get heterogeneous band sizes.  look for the right size.





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