Dear All,
My lab is planning to synthesize some carrier RNA (poly-A) to increase the yield of (viral) DNA precipitation. Does the length of poly-A RNA matters? What should be the best length to be synthesized? Thanks all!
Synthesis of carrier RNA(poly-A) for DNA Extraction
Started by GordonLeung, Sep 19 2012 05:20 AM
DNA extraction carrier RNA optimization oligo synthesis
2 replies to this topic
#1
Posted 19 September 2012 - 05:20 AM
#2
Posted 20 September 2012 - 05:59 AM
GordonLeung, on 19 September 2012 - 05:20 AM, said:
Dear All,
My lab is planning to synthesize some carrier RNA (poly-A) to increase the yield of (viral) DNA precipitation. Does the length of poly-A RNA matters? What should be the best length to be synthesized? Thanks all!
My lab is planning to synthesize some carrier RNA (poly-A) to increase the yield of (viral) DNA precipitation. Does the length of poly-A RNA matters? What should be the best length to be synthesized? Thanks all!
yes it matters 35 to 40 datp is most suitable..
#3
Posted 20 September 2012 - 05:23 PM
Thanks Talha.
Also tagged with one or more of these keywords: DNA extraction, carrier RNA, optimization, oligo synthesis
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