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Synthesis of carrier RNA(poly-A) for DNA Extraction

DNA extraction carrier RNA optimization oligo synthesis

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#1 GordonLeung

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Posted 19 September 2012 - 05:20 AM

Dear All,

My lab is planning to synthesize some carrier RNA (poly-A) to increase the yield of (viral) DNA precipitation. Does the length of poly-A RNA matters? What should be the best length to be synthesized? Thanks all!

#2 talha

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Posted 20 September 2012 - 05:59 AM

Dear All,

My lab is planning to synthesize some carrier RNA (poly-A) to increase the yield of (viral) DNA precipitation. Does the length of poly-A RNA matters? What should be the best length to be synthesized? Thanks all!


yes it matters 35 to 40 datp is most suitable..

#3 GordonLeung

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Posted 20 September 2012 - 05:23 PM

Thanks Talha. :D





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