Does triple labeled protein precipitate more easily?
Posted 19 September 2012 - 04:52 AM
I purified two different proteins these days. Both were labeled with 2H, 15N and 13C. I found they were light yellow when concentrated. However, the same unlabeled proteins I had purified previously were colorless， even though concentrated to a higher concentration. I don't know why and I am wondering whether triple labeled proteins are more likely to precipitate. Is there anyone can help with my questions? Thank you very much!
Posted 19 September 2012 - 01:58 PM
Posted 19 September 2012 - 05:13 PM
I don't think addition of different isotopes will change the precipitability substantially. The colour is more likely to be from a buffer or something in the prep (detergents?) oxidizing.
Thank you! But only one of the buffer contains detergent (DPC), the other doesn't.