here is my first try WB with blood serum.http://imageshack.us.../membraneh.jpg/
From picture above,the first 4 lanes are normal sample from the whole cell extraction, in lane 5-8 are my first test blood serum.
Total protein I loaded is 10 ug (1ug/uL) in all samples. For the last 2 is my positive control.
Could someone give me some advice if what is commonly use for internal standard. I normaly use beta actin 43kDa but in the srerum sample, It seems not work.
Albumin seems to be too high, or not ?, because I'm plaining to increase total protein concentration for the next time.
I h..ve ne..er finish.. ...ny thi .....