After I pour my LB agar into the plates, we always get condensation on the lid.
Does anyone have any advice for this not to happen?
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How to not get condensation on lid of agar plates?
Started by Smog187, Sep 18 2012 11:30 AM
Agar plates
4 replies to this topic
#2
hobglobin
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Posted 18 September 2012 - 11:33 AM
while cooling and solidifying you can leave the lid a bit open under the clean bench
more or less like in this not good picture:
more or less like in this not good picture:
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
#3
phage434
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Posted 18 September 2012 - 03:26 PM
You can reduce this by putting an empty petri dish on top of the stack. Not a complete solution. Also, make sure you are pouring the plates after the agarose is cooled to 53C or so (not hotter).
#4
Bpaul
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Posted 18 September 2012 - 07:29 PM
We just keep the lid a bit open after pouring the agar! and we keep the plates open in the 37degC incubator for a while till everything dries!
#5
leelee
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Posted 19 September 2012 - 04:27 AM
I do as phage434 suggests, pouring the agar only once the bottle is cool enough to hold comfortably in an ungloved hand.
I then leave them overnight on the bench, and the next day any condensation is gone.
If I need one that has been in the fridge, I take it out several hours before I need to use it and leave it either on the bench or if it is a cooler day, then slightly open in a tissue culture hood, or 37C warm room (upside down if in the warm room).
I then leave them overnight on the bench, and the next day any condensation is gone.
If I need one that has been in the fridge, I take it out several hours before I need to use it and leave it either on the bench or if it is a cooler day, then slightly open in a tissue culture hood, or 37C warm room (upside down if in the warm room).
