Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Peptide concentration methods

peptideprecipitation; concentration; pichia pastori;

  • Please log in to reply
No replies to this topic

#1 Clauelena13



  • Members
  • Pip
  • 2 posts

Posted 18 September 2012 - 10:42 AM

I have been trying to produce a 6kDa peptide through two different strategies. One of them is using Pichia pastori secretory production. In this case, the problem is that I cannot detect the 6kDa peptide in the media...it could be that the pichia clones obtained are not good in terms of production, but to considere this I first have to discard that the peptide is been produced in levels only seen when concentrating the media...have you any ideas about peptide concentration methods using in the broth media? I believe that ultrafiltration is not suitable because of large volumes I need to use...What about precipitation methods? HAve you any experience in this point? Iam not sure if TCA precipitation works well with peptides.
Thanks in advanced,

Also tagged with one or more of these keywords: peptideprecipitation;, concentration;, pichia pastori;

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.