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Peptide concentration methods

peptideprecipitation; concentration; pichia pastori;

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#1 Clauelena13

Clauelena13

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Posted 18 September 2012 - 10:42 AM

I have been trying to produce a 6kDa peptide through two different strategies. One of them is using Pichia pastori secretory production. In this case, the problem is that I cannot detect the 6kDa peptide in the media...it could be that the pichia clones obtained are not good in terms of production, but to considere this I first have to discard that the peptide is been produced in levels only seen when concentrating the media...have you any ideas about peptide concentration methods using in the broth media? I believe that ultrafiltration is not suitable because of large volumes I need to use...What about precipitation methods? HAve you any experience in this point? Iam not sure if TCA precipitation works well with peptides.
Thanks in advanced,
Clau





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