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Single Plasmid Gel Band

electrophoresis gel band non-coiled special conditions

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#1 Otto Heringer

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Posted 18 September 2012 - 09:36 AM

Can (in any misterious special conditions) a non-digested plasmid appear as a single band (without the coiled states) in a ordinary agarose electrophoresis gel!?
I heard this rumor by a colleague and didn't found anything consistent in the literature.

Personally, I think that it not make much sense. Any thoughts or experiences!?

Thanks!

#2 bob1

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Posted 18 September 2012 - 01:05 PM

Sure, if the plasmid wasn't prepared properly. Dring alkaline lysis it can be nicked by the conditions, shearing of the DNA by over-vigorous shaking, vortexing or pipetting can also damage the DNA.

It is pretty uncommon though, I would still expect to see both nicked and linear forms.





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