Can (in any misterious special conditions) a non-digested plasmid appear as a single band (without the coiled states) in a ordinary agarose electrophoresis gel!?
I heard this rumor by a colleague and didn't found anything consistent in the literature.
Personally, I think that it not make much sense. Any thoughts or experiences!?
Thanks!
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Single Plasmid Gel Band
Started by Otto Heringer, Sep 18 2012 09:36 AM
electrophoresis gel band non-coiled special conditions
1 reply to this topic
#2
bob1
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Thelymitra pulchella
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Posted 18 September 2012 - 01:05 PM
Sure, if the plasmid wasn't prepared properly. Dring alkaline lysis it can be nicked by the conditions, shearing of the DNA by over-vigorous shaking, vortexing or pipetting can also damage the DNA.
It is pretty uncommon though, I would still expect to see both nicked and linear forms.
It is pretty uncommon though, I would still expect to see both nicked and linear forms.
