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Cell plating

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#1 vjlj

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Posted 18 September 2012 - 07:40 AM

Hi all,

I  got 6.75 number of cells  which is  average of 4  squares and my  dilution factor is 1.
I would like to plate one plate with 12 wells and per well i  need 3.5 x 10 to  power of 4 cells/well.
Can u tell me how much of cells stocks wil  be used /well.

#2 casandra

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Posted 18 September 2012 - 09:55 AM

You took the average of the 4 big (primary) squares? And the concentration of your stock is 6.75 x 105 or106 ....cells/ml? (for hemocytometer counting, here’s a good primer: http://www.microbehu...unting-chamber/)

If you got your count right, then you can just use this straightforward formula:

C1V1=C2V2,

You already know your initial stock concentration (C1), your final conc C2 is 3.5 x 104/ml, V2 is 12 ml assuming that you would put 1 ml in each well so you can easily calculate how much of the stock you need to add to a final volume of 12 ml.

Edited by casandra, 18 September 2012 - 10:02 AM.

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#3 vjlj

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Posted 18 September 2012 - 08:49 PM

Thank you  cassandra..:-)