Posted 18 September 2012 - 07:40 AM
I got 6.75 number of cells which is average of 4 squares and my dilution factor is 1.
I would like to plate one plate with 12 wells and per well i need 3.5 x 10 to power of 4 cells/well.
Can u tell me how much of cells stocks wil be used /well.
Posted 18 September 2012 - 09:55 AM
If you got your count right, then you can just use this straightforward formula:
You already know your initial stock concentration (C1), your final conc C2 is 3.5 x 104/ml, V2 is 12 ml assuming that you would put 1 ml in each well so you can easily calculate how much of the stock you need to add to a final volume of 12 ml.
Edited by casandra, 18 September 2012 - 10:02 AM.
- hobglobin, personal comment about my beauteous photo......